[Hot Item] Dextran Gel Filtration Chromatography Equal to Sephadex G-100
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[Hot Item] Dextran Gel Filtration Chromatography Equal to Sephadex G25
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Gel Filtration Chromatography • Microbe Online
Gel Filtration Chromatography • Microbe Online
May 19, 2022
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Question 24 ptsNext, the fractions containing PFK-1 activity were applied to a DEAE-Sephadex (anionexchange) column. The column was equilibrated with a pH = 8.2 buffer. The column waselutated with a salt (ammonium sulfate) gradient and the results are shown in Figure 1.Activity, Units/ml (102010203040506020406080100Fraction NumbermM (NH4)2SO4 in Eluate (—)Figure 1. Purification of PFK-1C by DEAE Sephadex (anion-exchange) chromatography.S 1 2 3 4 S220116976843Figure 2. SDS-PAGE analysis of the PF
Question 24 ptsNext, the fractions containing PFK-1 activity were ap…
Aug 11, 2022
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