8.10: Sanger Sequencing of DNA
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SOLVED:Provide a brief summary of the Sanger sequencing method. a. Frederick Sanger’s sequencing is a chain termination method that is used to generate DNA fragments that terminate at different points using dye-labeled dideoxynucleotides. DNA is separated by electrophoresis on the basis of size. The DNA sequence can be read out on an electropherogram generated by a laser scanner. b. Frederick Sanger’s sequencing is a chain elongation method that is used to generate DNA fragments that elongate at
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