Western Blotting Summary - BioTechniques
Western blotting (or immunoblotting) is a well-established biochemical technique that detects specific proteins in complex samples and has applications from basic research to the diagnosis of infectious diseases. Western blotting can be used to determine quantity, molecular weight and post-translational modifications of proteins and can ...
Metastasis-Associated Macrophages Discovered - BioTechniques
Sep 13, 2024 · Metastasis-associated macrophages identified. Several subsets of macrophage have been found to closely associate with metastasis-initiating cells in the tumor microenvironment. A team of researchers at the Johns Hopkins Kimmel Cancer Center (MD, USA) have used mass cytometry to identify a subset …
Home - BioTechniques
1 day ago · Explore BioTechniques. A world leader in life science method research news. ISSN (print): 0736-6205 | ISSN (online): 1940-9818.
The Brain’s Defense Against Herpes Encephalitis - BioTechniques
Aug 9, 2024 · The research, presented in two papers, provides a vital insight into a little known anti-viral immune response, providing a potential avenue for treatment of the rare instances that herpes can infect the brain and other neurological infections. Herpes simplex virus 1 (HSV1) is capable of infecting nerve cells, and infections are near-ubiquitous ...
From Academia to Biotech with Bill Brizzard - BioTechniques
Mar 21, 2024 · Taking the leap: moving from academia to the biotech industry. Bill Brizzard (left; Brizzard Consulting LLC, MO, USA) made the move from academic researcher to biotech industry professional before becoming an independent consultant. In this interview, Brizzard provides insights into this transition, the importance of mentoring and networking ...
34th First Annual Ig Nobel Prize Winners - BioTechniques
Sep 13, 2024 · Forget the breathalyzer, Tess Heeremans, Antoine Deblais, Daniel Bonn and Sander Woutersen (all University of Amsterdam, Netherlands) won the Ig Nobel chemistry prize for using chromatography to separate drunk and sober worms. As chemists, these researchers view worms as polymers, which are therefore fair game for the chromatographer.
How To: Spike-In Normalization - BioTechniques
Sep 18, 2024 · Conduct thorough quality control of the spike-in. Measure the spike-in-to-target ratio for each sample by isolating and sequencing the unenriched input sample. Visually interrogate the ChIP-seq signal for the spike-in using a genome browser, alongside metagenome analysis and peak calling. Use spike-in material from a model species with an ...
SfN 2024 Top Picks - BioTechniques
Sep 23, 2024 · What’s coming up at SfN 2024? This year’s Society for Neuroscience (SfN) annual meeting, Neuroscience 2024, is taking place at McCormick Place in Chicago (IL, USA) from 5–9 October 2024. The meeting brings together neuroscientists from across the world to discover new ideas and share the latest discoveries and advances in the field.
How to Normalize qPCR Data? [Webinar] - BioTechniques
Normalization of qPCR data. Watch now! Comparison of expression data requires normalization. The optimum normalization method depends on sample type, with the most common being to normalize to reference genes. It is critical to select appropriate reference genes and validate them. However, there are cases when one fails to identify suitable ...
What is Western Blotting? The Story of the Western Blot
Jul 18, 2018 · The technique involves the identification and characterization of DNA-binding proteins using DNA probes (Southern) and protein blotting (western) and was first described in 1980. This method was published before the western blot. Yet, it still alludes to it in its name, reaffirming the popularity of the western blot.