ELECTRON MICROSCOPY OF EARLY CYTOPLASMIC CHANGES DUE TO INFLUENZA VIRUS ...
Recently improved methods for visualization of thin tissue sections by electron microscopy have been applied to the study of early changes in the bronchial epithelium of mice infected by inhalation of aerosols of influenza virus.
Electron microscopy of influenza virus - ScienceDirect
Aug 20, 1985 · An electron microscopical study was made of the influenza virus, type B/Hong Kong, in the unstained, frozen, hydrated state after quench-freezing in cooled liquid ethane. The results are compared with data from negatively stained specimens.
Cryo-EM structure of influenza helical nucleocapsid reveals NP …
Dec 15, 2023 · In this study, we established a biological tool, the RNP-like particles assembled from recombinant influenza A virus NP and synthetic RNA, and we present the first subnanometric cryo–electron microscopy structure of the helical NP-RNA complex (8.7 to 5.3 Å).
Influenza virus-mediated membrane fusion: Structural insights …
Sep 1, 2015 · In this review, we survey EM-based contributions towards the characterization of influenza virus-mediated membrane fusion and anticipate the potential for future developments. Keywords: Cryo-electron microscopy; Cryo-electron tomography; Hemagglutinin; Influenza virus; Subtomogram averaging; Viral fusion.
Influenza Virus: Methods and Protocols | SpringerLink
Chapters detail a broad range of methods from diagnosis, virus propagation, proteomics, haploid screening, lentiviral screening, virus entry, single molecule RNA imaging, correlative light and electron microscopy (CLEM), EM, light-sheet microscopy, biochemistry, viral transcription, physiological infection models, animal models, in vivo imaging ...
Influenza virus-mediated membrane fusion: Structural insights …
Sep 1, 2015 · Briefly, cryo-electron microscopy (cryo-EM) and cryo-electron-tomography (cryo-ET) have shown the structure of influenza virions at neutral pH in close-to-native conditions; thin section EM has shown that influenza viruses enter the cell by endocytosis; negative staining and cryo-EM have shown the changes in the HA glycoproteins when virions ...
Influenza virus pleiomorphy characterized by cryoelectron tomography - PNAS
Dec 12, 2006 · For electron microscopy, the virus was further concentrated by pelleting (137,000 × g for 2 h) and resuspended at ≈6 mg/ml protein in 20 mM Hepes/20 mM Mes/130 mM NaCl, pH 7.5.
Structural transitions in influenza haemagglutinin at …
May 27, 2020 · Cryo-electron microscopy studies of the influenza haemagglutinin glycoprotein at the low pH of host endosomes reveals structural intermediates, offering a dynamic view of how the protein...
Correlative Light and Electron Microscopy of Influenza Virus
Aug 28, 2018 · The light microscopy stage enables visualization of fluorescently tagged viral particles and allows study of dynamic or rare intracellular trafficking events, while electron microscopy reveals the ultrastructural composition of the …
Electron microscopy of influenza virus: A comparison of …
Aug 20, 1985 · An electron microscopical study was made of the influenza virus, type B/Hong Kong, in the unstained, frozen, hydrated state after quench-freezing in cooled liquid ethane. The results are compared with data from negatively stained specimens.