eLife18m
Water and chloride as allosteric inhibitors in WNK kinase osmosensing
WNKs sense osmotic pressure by binding specific water molecules to the inactive dimeric configuration of WNK1 and WNK3, integrating signals with chloride inhibition as demonstrated by mutagenesis and ...
eLife3d
Single cell transcriptomics reveals early photoreceptor states, cell-specific transcript isoforms, and cancer-predisposing features
Here, we used deep full-length single-cell RNA-sequencing to distinguish post-mitotic cone and rod developmental states and cone-specific features that contribute to retinoblastomagenesis. The ...
eLife2d
Eed controls craniofacial osteoblast differentiation and mesenchymal proliferation from the neural crest
In this valuable study, the authors used an elegant genetic approach to delete EED at the post-neural crest induction stage. The usage of the single-cell RNA-seq analysis method is extremely suitable ...
eLife3d
Increased bone inflammation in type 2 diabetes and obesity correlates with Wnt signaling downregulation and reduced bone strength
Type 2 diabetes (T2D) and obesity (OB) are associated with chronic low-grade inflammation and increased fracture risk. In vitro studies showed that inflammation induces bone erosion and inhibits bone ...
eLife4d
The eLife Model: An update on progress following changes in Web of Science indexing status
Following the decision that eLife will not receive an Impact Factor in 2025, we share an update on how our model is doing ...
eLife4d
CXXC-finger protein 1 associates with FOXP3 to stabilize homeostasis and suppressive functions of regulatory T cells
This study presents important findings on the role of CXXC-finger protein 1 in regulatory T cell gene regulation and function. The evidence supporting the authors' claims is solid, with mostly ...
eLife4d
Auditory Cortex Learns to Discriminate Audiovisual Cues through Selective Multisensory Enhancement
Multisensory object discrimination is essential in everyday life, yet the neural mechanisms underlying this process remain unclear. In this study, we trained rats to perform a two-alternative ...
eLife6d
Development of a new genotype–phenotype linked antibody screening system
A Golden Gate-based dual-expression vector enables rapid screening, which facilitates efficient isolation of high-affinity cross-reactive antibodies for therapeutic or diagnostic use and provides a ...
eLife4d
Microbiome: Balancing microbial composition through diet
The gut of animals is a complex ecosystem consisting of trillions of bacteria and other microorganisms that are unique to every individual. Infants can inherit their first gut bacteria through vaginal ...
eLife3d
Emergence of alternative stable states in gene exchange communities
This manuscript offers a modeling platform in which horizontal gene transfer (HGT) is incorporated into the ecological dynamics of microbial communities. The investigation is valuable as it brings to ...
eLife6d
The ESCRT protein CHMP5 restricts bone formation by controlling endolysosome-mitochondrion-mediated cell senescence
In this study, our results uncover the function and mechanism of CHMP5 in the regulation of cell senescence and bone formation in osteogenic cells. Deletion of Chmp5 causes endolysosomal dysfunction ...
eLife6d
Molecular dynamics simulations illuminate the role of sequence context in the ELF3-PrD-based temperature sensing mechanism in plants
In this potentially valuable computational study, the authors conducted atomistic and coarse-grained simulations to probe the temperature-dependent phase behaviors of ELF3, a disordered component of ...