Holoenzyme was visualized by immunoblot analysis using a pol II CTD specific antibody. Lane 1 represents 10% of total RHA or holoenzyme. c, Mapping of the RHA binding domain of BRCA1.

Mingqiu Dai, Chen Zhang, Urszula Kania, Fang Chen, Qin Xue, Tyra Mccray, Gang Li, Genji Qin, Michelle Wakeley, William Terzaghi, Jianmin Wan, Yunde Zhao, Jian Xu, Jiří Friml, Xing Wang Deng, Haiyang ...

Zafar Zaman, Aseem Z. Ansari, Sang Seok Koh, Richard Young, Mark Ptashne, Interaction of a Transcriptional Repressor with the RNA Polymerase II Holoenzyme Plays a Crucial Role in Repression, ...

Using cryo-microscopy, researchers present a high-resolution structure of monkeypox virus DNA polymerase holoenzyme, a complex that plays a key role in the genome replication process of the virus.

They determined the holoenzyme structure in complex with DNA using cryo-electron microscopy at the global resolution of ~2.8 Å. According to the findings, the holoenzyme possesses an architecture ...